Vitreous Cryopreservation of Human Preantral Follicles Encapsulated in Alginate Beads with Mini Mesh Cups

DOI Web Site Web Site PubMed 参考文献88件
  • BIAN Jiang
    Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, P. R. China Department of Obstetrics and Gynecology, Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200233, P. R. China
  • LI Tao
    Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, P. R. China
  • DING Chenhui
    Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, P. R. China
  • XIN Weijie
    Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, P. R. China
  • ZHU Bo
    Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, P. R. China
  • ZHOU Canquan
    Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, P. R. China

この論文をさがす

抄録

To completely avoid ice crystal formation and thus get a higher survival rate, vitrification methods have been commonly used for cryopreservation of oocytes and embryos. However, currently used vitrification methods for oocytes and embryos are not suitable for the cryopreservation of preantral follicles (PFs). In the present study, stainless steel mesh was fabricated into mini mesh cups to vitrify isolated PFs. Moreover, isolated follicles were encapsulated and then subjected to vitreous cryopreservation to facilitate in vitro culture/maturation of follicles after warming. The results showed that the percentages of viable follicles did not differ significantly between the vitrification group and fresh group soon after warming (81.25% vs. 85.29%, P>0.05) and after a 7-day culture period (77.78% vs. 83.33%, P>0.05). No difference in mean follicular diameter was observed between cryopreserved and fresh follicles when cultured in vitro. Transmission electron microscopic analysis revealed that vitreous cryopreservation could maintain the ultrastructure of follicles in alginate beads. In conclusion, the present vitrification method could efficiently cryopreserve isolated human ovarian follicles encapsulated by calcium alginate, which could be put into immediate use (in vitro culture/ maturation) after warming. However, more follicles and some detailed biochemical analyses are required to further investigate the effects of vitrification on the long-term growth of human encapsulated PFs.

収録刊行物

参考文献 (88)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ