DNA Polymerase ^|^lambda; Promotes Mutagenesis Induced by 8-Oxo-7,8-dihydroguanine (8-hydroxyguanine) Paired with Adenine

• Kamiya Hiroyuki

  Graduate School of Science and Engineering, Ehime University

• Kurokawa Masahiro

  Graduate School of Science and Engineering, Ehime University

書誌事項

タイトル別名

• DNA Polymerase λ Promotes Mutagenesis Induced by 8-Oxo-7,8-dihydroguanine (8-hydroxyguanine) Paired with Adenine

抄録

DNA polymerase λ preferentially inserts dCTP opposite 8-oxo-7,8-dihydroguanine (G^O, 8-hydroxyguanine) in vitro, and this function is considered to be important after A base removal from G^O:A pairs by the MUTYH DNA glycosylase. However, dCTP incorporation would promote A:T→C:G transversion mutations induced by 8-oxo-7,8-dihydro-dGTP (dG^OTP), the mutation triggered by the incorporation of dG^OTP opposite A in the template DNA. In this study, double-stranded plasmid DNA containing the G^O:A pair, an intermediate in the dG^OTP mutagenesis pathway, was transfected into human cells in which DNA polymerase λ was knocked down. The knockdown of DNA polymerase λ significantly reduced the frequency of A:T→C:G transversion mutations induced by the G^O:A pair although the knockdown effect was small. These results suggested that DNA polymerase λ is involved in the mutagenesis processes of G^O, generated by dG^OTP, in the nucleotide pool.<br>

収録刊行物

• Genes and Environment

  Genes and Environment 35 (4), 105-109, 2013

  日本環境変異原学会

キーワード

• 8-oxo-7,8-dihydroguanine
• 8-hydroxyguanine
• DNA polymerase λ
• siRNA
• knockdown

詳細情報

• CRID: 1390001205257244032
• NII論文ID: 10031196752
• NII書誌ID: AA1212552X
• DOI: 10.3123/jemsge.2013.002
• COI: 1:CAS:528:DC%2BC3sXhvVyhurvJ
• ISSN: 18807062
• ISSN: 18807046
• NDL書誌ID: 025004111
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025004111
• Web Site: https://search.jamas.or.jp/link/ui/2015031885
• 本文言語コード: en
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