Complete Nucleotide Sequence of Bacillus sub tilis (na tto) Plasmid Responsible for γ-Polyglutamate Synthesis

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The complete 5,812 base pairs nucleotide sequence has been determined for Bacillus subtilis (natto) plasmid pUH1, which encodes a ymglutamyltranspeptidase (EC 2.3.2.2) for synthesis of y-polyglutamate. Nucleotide sequence analysis reveals two long open reading frames coding for the gene for replication (rep) protein and y-glutamyltranspeptidase gene. Several putative regulatory sequences conserved were found upstream from these frames including the ShinemDalgarno sequence, Pribnow box and the “-35 region” at preferred distances for efficient transcription. The amino acid sequences predicted from the rep and ymglutamyltranspeptidase coding frames showed a significant homology, in each small segment, with those of E229 coded from the plasmid PC194 and of C403 from the plasmid pE194 of Staphylococcus aureus, respectively. However, no convincing homology of the pUH1 predicted ymglutamyltranspeptidase protein could be found with E. coli and mammalian ymglutamyltranspeptidases.

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詳細情報 詳細情報について

  • CRID
    1390572174637669120
  • NII論文ID
    110000017712
  • NII書誌ID
    AA00247166
  • DOI
    10.5109/23986
  • HANDLE
    2324/23986
  • ISSN
    00236152
    http://id.crossref.org/issn/00236152
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • IRDB
    • Crossref
    • CiNii Articles

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