肝転移を抑制する漢方方剤における活性酸素消去能からみた特徴  [in Japanese] Inhibitory effects on superoxide anion radical and hydroxyl radical by Chinese herbal with the preventive function for experimantal hepatic metastasis  [in Japanese]

十全大補湯, 四物湯, 補中益気湯, 小柴胡湯の経口投与は実験的ラット肝転移を抑制し, 十全大補湯と四物湯はマクロファージを, 補中益気湯はNK細胞を活性化するが, これら漢方方剤における活性酸素消去能の特徴を検討した.上記を含めて9種類の漢方方剤を検討したところ, ・O_2^-と・OH(Fenton反応・UV照射)の消去能の総和では小柴胡湯が最も強く, 温清飲, 補中益気湯十全大補湯四物湯人参養栄湯の順であった.更にこれら9種類の構成生薬23種類別における活性酸素消去能の検討の結果, 漢方方剤の特徴として十全大補湯, 四物湯温清飲は・O_2^-を, 補中益気湯と小柴胡湯は・OHを抑える傾向がみられ, ・O_2消去能の高い漢方方剤はマクロファージ活性に, ・OH消去能の高い漢方方剤はNK細胞活性に関与するという可能性が示唆された.

Oral administration of Juzen-taiho-to , Shimotsu-to, Hochu-ekki-to, and Sho-saiko-to inhibits experimental liver metastasis. Juzen-taiho-to and Shimotsu-to activate macrophages, and also Hochu-ekki-to and Sho-saiko-to activate NK cells. The components that activate macrophages and NK cells in these Chinese herbal medicine (Juzen-taiho-to, Shimotsu-to, Shikunshi-to, Rikkunshi-to, Unsei-in, Ninjin-yoei-to, Toki-shakuyaksan, Hochu-ekki-to, and Sho-saiko-to) were examined for scavenging activity of free radicals. In 9 Chinese herbal medicine, the total intensity of scavenging activity on・O_2^-(HPX-XOD reaction) and・OH (Fenton Reaction, Ultra-Violet product) was Sho-saiko-to > Unsei-in > Hochu-ekki-to > Juzen-taiho-to > Shimotsu-to > Ninjin-yoei-to. In 23 crude components of 9 Chinese herbal medicines, ・O_2^- scavenging activity was highest in Jio, and ・ OH scavenging activity was highest in Kanzou. In conclusion, the potential for macrophage activation was indicated for components with a high ・ O_2^- scavenging ability, and NKcell activation was indicated for components with a high・OH scavenging ability.