共焦点レーザー走査顕微鏡と光学顕微鏡を組み合わせた免疫組織化学法によるハト視床下部漏斗部のVIPニューロンとGnRHニューロンの形態解析

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  • Morphological relationship between VIP-and GnRH-like immunoreactive neurons by conforcal laser scanning and light microscopic analyses in the hypothalamic infundibular region of the pigeon
  • キョウ ショウテン レーザー ソウサ ケンビキョウ ト コウガク ケンビキョウ オ クミアワセタ メンエキ ソシキ カガクホウ ニ ヨル ハト シショウカブ ロウト ブ ノ VIP ニューロン ト GnRH ニューロン ノ ケイタイ カイセキ

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The hypothalamic infundibular region of the birds comprise neuronal elements immunoreactive (ir) or vasoactive intestinal polypeptide (VIP) and gonadotropin-releasing hormone (GnRH). By use of immunohistochemical double-labeling techniques, we have investigated the morphological relationships between these two types of peptidergic neuronal elements in the infundibular area of the pigeon at light microscopic levels. Serial sectioned images of conforcal laser scanning microscopy revealed that GnRH-like-ir fibers or button-like structures were in close apposition to VIP-like-ir cell bodies or processes (probably dendritic processes). These results were confirmed by a method for combining conforcal and conventional light microscopic examinations of Vibratom sections processed for double-labeling immunohistochemistry. This method showed that these close apposition of two types of peptidergic neuronal elements were observed in a sudependymal area of infundibular region dorsal to the median eminence. These data suggest that GnRH fibers innervate shbependymal VIP neurons of the infundibular region near the median eminence. The infundibular regions of the bird are possible sites of interaction between VIP and GnRH neuronal systems.

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