<原著>螢光in situハイブリダイゼーション法による高精度ヒト1番染色体短腕の細胞遺伝学的地図の作製と1p36.1領域のコスミッドマーカーの配列決定

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  • <Originals>Construction of high-resolution cytogenetic map of the short arm of human chromosome 1 bearing the precise ordering of 18 DNA markers on 1p36.1 using fluorescence in situ hybridization

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Based on observations of chromosomal aberration, genes that are associated with several types of human tumors (leukemia, neuroblastoma, melanoma, or cancers of colon, stomach and breast, etc.) are located on the short arm of chromosome 1. A high-resolution cytogenetic map of the short arm of human chromosome 1 with newly isolated 411 cosmid markers was constructed by fluorescence in situ hybridization (FISH). These markers were scattered throughout chromosome 1p, but they were preferentially concentrated on the R band dominant regions such as 1p36,1p34,1p32,1p22,and 1p13,especially on the T-band region (1p36-p34). Among these markers, 197 were localized on chromosome band 1p36,a region frequently deleted in neuroblastoma and malignant melanoma. Of these, 18 were precisely ordered on 1p36.1 by multi-color prophase FISH ordering system and stretched DNA FISH as follows ; 1cen-(1p36.1)-652-176-74-241-561-190-199-451-665-631-614-568-586-226-1-11-41-163-1pter. This high-resolution map of chromosome 1p can provide useful information and landmarks for the human genome project and expedite the identification of breakpoints and/or tumor suppressor gene(s) associated with several types of malignant tumors that frequently exhibit chromosomal aberrations or deletions of chromosome 1p.

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