A Family of Highly Repeated DNA Sequences of the Fern Sawfly Hemitaxonus japonicus Complex(Hymenoptera:Tenthredinidae)

• SONODA S.

  Laboratory of Applied Entomology, Faculty of Agriculture, Okayama University

• YAMADA Tetsuji

  Laboratory of Plant Pathology, Faculty of Agriculture, Okayama University

• NAITO Tikahiko

  Entomological Laboratory, Faculty of Agriculture, Kobe University

• NAKASUJI Fusao

  Laboratory of Applied Entomology, Faculty of Agriculture, Okayama University

抄録

A family of highly repeated DNA sequences of the fern sawfly, Hemitaxonus japonicus (Polystichum tripteron race) has been cloned. Hybridization analysis of the EcoRI, SacI and Sau3AI digests of the genomic DNA isolated from H. japonicus (Polystichum tripteron race) against the recombinant plasmid, pYS354, containing tandemly repeated sequences showed ladders of each basic monomer unit having a length of ca. 270-bp. In addition to pYS354, the other two types of clones that also contain tandemly repeated sequences isolated from H. japonicus (Polystichum tripteron race) were cloned and the nucleotide sequences were compared with pYS354. Nucleotide sequences of these repeated sequences showed high homology between each other with highly AT-rich sequences. The presence of the homologous tandemly repeated sequences in the genome of ten species belonging to six genera of Tenthredinidae was analyzed by hybridization against pYS354 as a probe. Among them, only PP-race, the oligophagous population of H. japonicus and the sibling species, H. sasayamensis, possessed such tandemly repeated DNA sequences.

収録刊行物

• Appl.Entomol.Zool.

  Appl.Entomol.Zool. 27 399-405, 1992

  日本応用動物昆虫学会

キーワード

• repeated DNA
• tandem repeat
• Hemitaxonusjaponicus
• Tenthredinidae
• DNA sequence

詳細情報

• CRID: 1572543026772705280
• NII論文ID: 110001105301
• NII書誌ID: AA00543238
• ISSN: 00036862
• 本文言語コード: en
• データソース種別:
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