Gingival Fibroblasts Inhibit Mineralized Bone Nodule Formation by Alveolar Bone-derived Cells In Vitro :

NDLデジタルコレクション 被引用文献2件
  • Suzuki,Naoto
    Department of Biochemistry, Nihon University School of Dentistry
  • Oikawa,Eiji
    Department of Biochemistry, Nihon University School of Dentistry
  • Ogawa,Hidenori
    Department of Biochemistry, Nihon University School of Dentistry
  • Muto,Akira
    Department of Biochemistry, Nihon University School of Dentistry
  • Fujikaa,Kenji
    Department of Periodontology,Nihon University School of Dentistry
  • Maeno,Masao
    Department of Biochemistry, Nihon University School of Dentistry
  • Otsuka,Kichibee
    Department of Biochemistry, Nihon University School of Dentistry

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The errects of the conditioned culture medium from rabbit gingival fibroblasts (GF) on minera1ized nodule formation by rabbit alveolar bone-derived (AB) cells were investigated using confluent cultured AB cells. The secreted proteins in the conditioned media from both types of cell were determined using sodium dodecyl sulrate (SDS)-polyacrylamide gel electrophorcsis (SDS-PAGE). The cells were cultured in α-minimum essential medium (α-MEM) with and without [14C1proline and l% (vol/vol) serum for 24h. The protein bands in the GF conditioned medium (GFCM) separated by SDS-PAGE showed it contained larger amounts of proteins with molecular masses of over 200 and 50-70kDa, and yielded higher quantities of [14C] pro1ine-1ncorporated proteins with molecular masses of over 200 and 67 kDa than AB cells. Confluent AB cells were cultured in GFCM diluted with an equal volume of α-MEM supplemented with serum and ascorbic acid for 14 days and the mineralized and unmineralized nodules that fornled were observed with the naked eye and counted. The addition of 50% (vol/vol) GFCM to the AB cell cultures reduced the number of minera1ized nodules significantly. The GFCM was divided into 4 fractions (pools 1 to 4), depending on their molecular masses, using gel-filtration column chromatography. Mineralized nodule formation by AB cells was reduced significantly by adding pools 2 and 3, wllich contained proteins with molecular masses of 67-120 and 13-67 kDa respectively, to the culture. Pool 3 was the most effective inhibitor of nodule minera1ization. These results suggest that GF may coordinate osteogenesis of AB cells via the release of some soluble inhibitory factors with a molccular masses of 50-67 kDa.

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