Highly Effective Method for Collecting Autologous Peripheral Stem Cells from Patients with Multiple Myeloma

• Yasuda Masahiro

  Gifu Municipal Hospital, Department of Pharmacy

• Sakaida Masamitsu

  Gifu Municipal Hospital, Department of Pharmacy

• Kato Hiroaki

  Gifu Municipal Hospital, Department of Pharmacy

• Yokoyama Yuko

  Gifu Municipal Hospital, Department of Clinical Laboratory

• Tanahashi Junko

  Gifu Municipal Hospital, Department of Nursing

• Shimomura Yoriko

  Gifu Municipal Hospital, Department of Hematology

• Takahashi Takeshi

  Gifu Municipal Hospital, Department of Hematology

Bibliographic Information

Other Title

• 多発性骨髄腫患者における自己末梢血幹細胞の高効率な採取法

Abstract

We investigated a highly effective method for collecting a sufficient quantity of autologous peripheral stem cells from patients with multiple myeloma in a single attempt. Eight patients (5 males and 3 females, mean age : 56.5 years) underwent remission-induction chemotherapy (built around a VAD regimen), followed by mobilization chemotherapy using cyclophosphamide (CPA 2 g/m² given for 2 days, 7 patients) or etoposide (VP-16, 500 mg/m² for 3 days, one patient). When the leukocyte count dropped below 1, 000/μL, a granulocyte colony stimulating factor, lenograstim (7.1 ±2.9μg/kg/day), was administered for around 7 days. When the leukocyte count exceeded 10⁴/μL, stem cells were collected by processing the blood in a fluid having a volume of three times that of circulating blood and using a COBE Spectra, a blood component separating device. The required number of hematopoietic stem cells (CD 34+ cells, over 2.0 × 10⁶/kg) was obtained from 6 patients in a single operation (within 4 hours). From 5 of them, a sufficient quantity was obtained for tandem grafting. Through adequate drug monitoring from the standpoint of a pharmacist, no severe adverse effects that would have necessitated interrupting the cell collecting procedure occurred.

Journal

• Iryo Yakugaku (Japanese Journal of Pharmaceutical Health Care and Sciences)

  Iryo Yakugaku (Japanese Journal of Pharmaceutical Health Care and Sciences) 30 (4), 280-287, 2004

  Japanese Society of Pharmaceutical Health Care and Sciences

Keywords

• autologous peripheral blood stem cell harvest (auto-PBSCH)
• multiple myeloma
• lenograstim
• cyclophosphamide (CPA)
• VAD therapy

Details

• CRID: 1390282679751377024
• NII Article ID: 110001171525
• NII Book ID: AA11527197
• DOI: 10.5649/jjphcs.30.280
• ISSN: 18821499
• ISSN: 1346342X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10558185
• Web Site: https://search.jamas.or.jp/link/ui/2004219239
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • NDL-Digital
  • CiNii Articles
• Abstract License Flag: Disallowed