Isolation and Detection of Methanogens from the Gut of Higher Termites

• Deevong Pinsurang

  Environmental Molecular Biology Laboratory, RIKEN Department of Microbiology, Kasetsart University

• Hattori Satoshi

  Environmental Molecular Biology Laboratory, RIKEN

• Yamada Akinori

  Environmental Molecular Biology Laboratory, RIKEN Center for Ecological Research, Kyoto University

• Trakulnaleamsai Savitr

  Department of Microbiology, Kasetsart University

• Ohkuma Moriya

  Environmental Molecular Biology Laboratory, RIKEN PRESTO, Japan Science and Technology Agency

• Noparatnaraporn Napavarn

  Department of Microbiology, Kasetsart University

• Kudo Toshiaki

  Environmental Molecular Biology Laboratory, RIKEN Graduate School of Integrated Science, Yokohama City University

抄録

Four strains of hydrogenotrophic methanogens showing narrow ranges of utilizable substrates were isolated in pure cultures from the guts of various feeding groups of (phylogenetically) higher termites. An analysis of the 16S rRNA gene sequence revealed that three strains were closely related to Methanobacterium bryantii (>99% nucleotide identity), the other to the genus Methanobrevibacter. The latter was related to a clone identified previously from the gut of a higher termite without cultivation (clone MPn19) and Methanobrevibacter arboriphilicus (99.0 and 97.9% nucleotide identity, respectively) but distinct from the species identified in lower termites. The specific detection of related methanogens in the gut population by nested-PCR indicated that every termite harbored the species of Methanobrevibacter. However, the methanogen related to the Methanobacterium strain was not detected in two termite species from which the Methanobacterium strains were isolated, suggesting that they are less prevalent in the gut community.<br>

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 19 (3), 221-226, 2004

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• <i>Methanobrevibacter</i>
• <i>Methanobacterium</i>
• symbiosis
• termite

詳細情報

• CRID: 1390282679323818496
• NII論文ID: 110001269380
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.19.221
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 7097682
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7097682
• Web Site: http://www.jstage.jst.go.jp/article/jsme2/19/3/19_3_221/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可