Horizontal Transfer of Nodulation Genes in Soils and Microcosms from Bradyrhizobium japonicum to B. elkanii.

• Minamisawa Kiwamu

  Graduate School of Life Sciences, Tohoku University

• Itakura Manabu

  Graduate School of Life Sciences, Tohoku University

• Suzuki Masako

  School of Agriculture, Ibaraki University

• Ichige Kazunori

  School of Agriculture, Ibaraki University

• Isawa Tsuyoshi

  Graduate School of Life Sciences, Tohoku University School of Agriculture, Ibaraki University

• Yuhashi Ken-ichi

  Graduate School of Life Sciences, Tohoku University School of Agriculture, Ibaraki University Plant Biotechnology Institute, Ibaraki Agriculture Center

• Mitsui Hisayuki

  Graduate School of Life Sciences, Tohoku University

Abstract

We investigated the horizontal transfer of nodulation (nod) genes to a Bradyrhizobium elkanii strain, lacking common nod genes as a recipient, in soils and microcosms using selection systems of antibiotic resistance and legume nodulation. We observed the horizontal transfer of nod genes at 4C in Nakazawa soil where peculiar strains (HRS strains) of B. japonicum harboring high copy numbers of insertion sequences dominated. In microcosms containing HRS strains as donors, we detected a similar horizontal transfer from B. japonicum HRS strain NK5 to the B. elkanii recipient more efficiently at 4C, which was verified by examining hybridization, nodulation and Nod factor production. These traits were, however, gradually lost during successive cultures. Plasmid analysis indicated that this event was not due to the simple transfer of plasmid carrying common nod genes. These results suggest the potential for horizontal transfer of nod genes among bradyrhizobia and other bacterial populations in soil environments.<br>

Journal

• Microbes and Environments

  Microbes and Environments 17 (2), 82-90, 2002

  Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Keywords

• horizontal gene transfer
• nodulation gene
• <i>Bradyrhizobium japonicum</i>
• <i>Bradyrhizobium elkanii</i>

Details

• CRID: 1390001204346115456
• NII Article ID: 110001271058
• NII Book ID: AA11551577
• DOI: 10.1264/jsme2.2002.82
• ISSN: 13474405
• ISSN: 13426311
• HANDLE: 10097/52074
• NDL BIB ID: 6206076
• Web Site: https://tohoku.repo.nii.ac.jp/records/41920
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6206076
• Web Site: http://www.jstage.jst.go.jp/article/jsme2/17/2/17_2_82/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed