Rapid DNA Extraction from a Bloom-forming Cyanobacterium.

• KONDO RYUJI

  Department of Marine Bioscience, Fukui Prefectural University

• KAGIYA GO

  Department of Marine Bioscience, Fukui Prefectural University

• HIROISHI SHINGO

  Department of Marine Bioscience, Fukui Prefectural University

• WATANABE MASAYUKI

  Department of Botany, National Science Museum

• HATA YOSHIHIKO

  Department of Marine Bioscience, Fukui Prefectural University

抄録

A rapid small-scale DNA extraction method is described for the toxic and/or bloom-forming cyanobacterial genus Microcystis, producing enough genomic DNA for polymerase chain reaction (PCR) amplification. PCR templates from 43 Microcystis strains were extracted and analyzed by PCR amplification. Sonication was needed for some strains before extraction of DNA using InstaGene^TM Matrix with heat treatment at 100°C. DNAs extracted from all strains used in this study by this method could be used as templates for PCR amplification. Depending on the apparatuses used, DNA extraction, PCR amplification and agarose gel electrophoresis analysis can be carried out on about 50 samples of Microcystis one day.

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 14 (3), 157-161, 1999

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• DNA extraction
• PCR
• <i>Microcystis</i>
• cyanobacteria

詳細情報

• CRID: 1390001204345629056
• NII論文ID: 110001272843
• NII書誌ID: AA11173196
• DOI: 10.1264/jsme2.14.157
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 4864989
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4864989
• Web Site: http://www.jstage.jst.go.jp/article/microbes1996/14/3/14_3_157/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可