Purification and Characterisation of a Novel Agarase Secreted by a Marine Bacterium, Pseudoalteromonas sp. Strain CKT1.

• XAVIER CHIURA HIROSHI

  Department of Biology, Division of Natural Sciences, International Christian University Mitaka, Tokyo 181-8585, Japan

• KITA-TSUKAMOTO KUMIKO

  Marine Microbiology Division Ocean Research Institute, University of Tokyo 1-15-1, Minamidai, Nakano, Tokyo 164-8639, Japan

抄録

A marine bacterium which grows on agar as its sole carbon and energy source shows an agar liquefying ability, was isolated from sea mud and identified as Pseudoalteromonas sp.An extracellular agarase was purified to homogeneity through a four-step process:ultra-filtration(10kDa<enzyme<0.2μm), two series of DEAE-Sephacel chromatography, and Sephacryl S-300 gel filtration.The enzyme was estimated to have a molecular mass of 56kDa by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and gel filtration, and be composed of a 37kDa protein and 19kDa of sugar, namely a glycoprotein.The optimum conditions for its action were 30ºC, pH of 6.5-7.5, and 0mM NaCl.Km to agarose was determined as 6.28mM.The enzyme was active at 15-65ºC, pH 4.0-9.0 and 0-4, 000mM salt concentration(NaCl).It was found to be an endo-type agarase which hydrolyses agar and agarose to yield galactose, neoagarobiose and a trace amount of neoagarohexaose.In addition, the enzyme had both α- and β-galactosidase activities.

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 15 (1), 11-22, 2000

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• agarase with broad susceptibility
• α- and β-galactosidase
• marine bacterium
• high salt tolerance
• Pseudoalteromonas

詳細情報

• CRID: 1390282679321233280
• NII論文ID: 110001272864
• NII書誌ID: AA11173196
• DOI: 10.1264/jsme2.2000.11
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 5335618
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5335618
• Web Site: http://www.jstage.jst.go.jp/article/jsme2/15/1/15_1_11/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
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