書誌事項
- タイトル別名
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- 技法シリーズ 微生物生態学への変性剤濃度勾配ゲル電気泳動法の応用
- ギホウ シリーズ ビセイブツ セイタイガク エ ノ ヘンセイザイ ノウド コウバイ ゲル デンキ エイドウホウ ノ オウヨウ
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抄録
The use of Denaturing Gradient Gel Electrophoresis(DGGE)has been widely recognized in microbial ecology and its importance has increased recently.DNA extracted from the environment is amplified by PCR using a set of primers in which one of the two contains the 40 base GC-rich sequence at the 5′ -end prior to DGGE. The PCR amplified DNA fragment migrates in a DGGE gel depending on its melting behavior. Moreover, the DNA resolved by DGGE can be promoted for further analyses, such as hybridization and sequencing without any cloning step. For these reasons, PCR-DGGE is one of the powerful tools available for the detection and phylogenetic analysis of environmental microbes. Although this method is easy and reproducible, some knowledge and technique are required to obtain reliable data. This technical series introduces protocols for the PCR-DGGE method with the theory presented to beginners as comprehensibly as possible. In addition, we discussed the application and limitation of the PCR-DGGE approach to microbial ecology.
収録刊行物
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- Microbes and environments
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Microbes and environments 15 (1), 59-73, 2000
日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282679321497216
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- NII論文ID
- 110001272869
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- NII書誌ID
- AA11173196
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- ISSN
- 13474405
- 13426311
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- NDL書誌ID
- 5335668
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可