ヒトインスリノーマの初代培養

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  • Primary culture of human insulinoma.

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The authors confirmed the secretion of insulin and somatostatin from the primary culture of resected human insulinoma during the last 9 months. The cell clumps consisted of Langerhans's island-like and fibroblast-like cells. The fibroblast-like cells were proliferated during the first one to two months in the early period of culture, but the number of Langerhans's island-like cells was the same and morphological changes were not observed. However insulin secretion was maintained at about 500 μU/ml for 9 months. Moreover, somatostatin was secreted at about 100 pg/ml during these period.<BR>Insulin secretory granules were noted in cell clumps by Peroxidase-antiperoxidase staining method. It is interesting to note that insulin and somatostatin were highly secreted in the cultivation over this period.

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