培養中皮細胞の形態学的観察　Ｉ　　組織化学所見および走査電顕像について

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  • Characteristics of human mesothelial cells studied in vitro.
  • 第一報組織化学所見および走査電顕像について

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Tissue cultures from body fluids of three cases were studied to clarify morphological characteristics of mesothelial cells in an in vitro system. These materials were obtained from ascites of a patient that had hepatoma with liver cirrhosis, and pleural fluids of a patient with metastatic gastric cancer and one with malignant mesothelioma. Immunocytochemical, enzyme histochemical and scanning electron microscopical studies of the cultured cells revealed the following results:<BR>1) Proliferated cells in vitro were divided into three types; epithelial-like (E-type), fibroblast-like (F-type) and giant-cell types (G-type).<BR>2) With scanning electron microscopy, many microvilli and a few pinocytic vesicles were observed on the surface of cultured mesothelial cells. These findings were in contrast to free-floating mesothelial cells whose surfaces were mainly covered by vesicles or blebs. From the present results, it is suggested that the cultured mesothelial cells, which settled to the glass surface, regained microvilli and pinocytic vesicles like mesothelial cells lining the serous body cavities.<BR>3) The cells from a patient with malignant mesothelioma proliferated easily in vitro and piled up. They exhibited more prominent morphological atypism than normal mesothelial cells in tissue culture.

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