螢光抗体法による子宮頸癌の診断

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タイトル別名
  • Immunodiagnosis of the cervical cancer by means of fluorescent antibody technique
  • とくに特異抗原の所在部位について

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An investigation has been made on the intracellular localization of tumor specific antigen of the cervical cancer by means of immunofluorescent antibody technique.<BR>The cancerous and normal tissues obtained from the patients of cervical cancer and from the normal cervices were fractionated by the method of W.C. Schneider into nuclear, mitochondrial and microsomal fractions respectively.<BR>Rabbits were divided into 3 groups and immunized with each fraction. The antisera vs. each fraction of cancerous tissue were absorbed by the each fraction prepared from the normal cervical tissue. The specificities of these sera were determined by the Ouchterlony plate. On agar geldiffusion tests, the tumor specificities were present to anti-mitochondrial and anti-microsomal sera.<BR>Histological sections and vaginal smears taken from squamous cell carcinoma of the uterine cervix and from normal cervix were stained by Coons' indirect immunofluorescent method using anticancer mitochondrial and microsomal sera. In this test it was found that specific fluorescence was shown in the cytoplasm of cancer cells and no fluorescence was seen in normal cervical epithelial cells.<BR>Consequently, it is evident that the cancer specificity exists in mitochondrial and microsomal fractions and this procedure will prove of difinitive value in the diagnosis of the uterine cervix.

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