Cold- or Heat-Tolerance of Leaves and Roots in Perennial Ryegrass Determined by <SUP>1</SUP>H-NMR

• Iwaya-Inoue Mari

  Laboratory of Crop Science, Department of Plant Resources, Faculty of Agriculture, Kyushu University

• Matsui Rina

  Department of Plant Resources, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• Fukuyama Masataka

  Laboratory of Crop Science, Department of Plant Resources, Faculty of Agriculture, Kyushu University

抄録

Dynamic states of water in the leaves and roots of perennial ryegrass (Lolium perenne L.) exposed to cold and heat stresses were studied by using ¹H-NMR. NMR spin-lattice relaxation times (T₁) of leaves in Arrhenius plots linearly decreased but increased as temperature decreased below 0°C. However, spin-spin relaxation times (T₂) of the leaves increased as temperature decreased from 20 to -20°C. TheT₂ value of the long fraction (associated with vacuole) in leaves decreased to about 600μs at -25°C, but that of the short fraction was about 10μs, and the relative value of signal intensity of the long fraction decreased to about 0.2 at -25°C. The T₂ values of the two fractions in roots decreased to about 1ms at -10°C. Judging from T₂ and electrolyte leakage, both vacuolar and cytoplasmic compartments of leaves and roots froze at these temperatures. T₁ of the leaves decreased slightly as temperature increased from 20 to 40°C but greatly decreased as temperature increased further. On the other hand, T₁ of the roots decreased linearly as temperature increased from 20 to 50°C. The levels of electrolyte leakage from leaves exposed to the above heat stress was low suggesting that membrane was not severely injured. In conclusion, analysis of Arrhenius plots of T₁ and T₂ is a sensitive and non-invasive method to evaluate primary responses of perennial ryegrass organs to the temperature stresses.

収録刊行物

• Plant Production Science

  Plant Production Science 7 (2), 118-128, 2004

  日本作物学会

キーワード

• Arrhenius plots
• Cold stress
• Dynamic states of water
• Heat stress
• NMR relaxation times (<I>T</I><SUB>1</SUB>, <I>T</I><SUB>2</SUB>)
• Organ
• Perennial ryegrass
• Water compartment

詳細情報

• CRID: 1390001204467097856
• NII論文ID: 110001720519
• NII書誌ID: AA11162156
• DOI: 10.1626/pps.7.118
• ISSN: 13491008
• ISSN: 1343943X
• Web Site: http://www.tandfonline.com/doi/pdf/10.1626/pps.7.118
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可