わが国オオムギ品種における硝酸イオン吸収のミハエリス＝メンテン式による解析

• 長谷川 博

書誌事項

タイトル別名

• Michaelis-Menten Kinetics of Nitrate Uptake in Japanese Barley Cultivars.
• ワガクニ オオムギ ヒンシュ ニ オケル ショウサン イオン キュウシュウ ノ

抄録

Nitrate uptake ability from low-concentration nitrate solution (below 1 mM) was evaluated based on Michaelis-Menten kinetics in 12 Japanese barley cultivars. Two kinetic parameters, Km and Vmax, were determined using 7-day-old seedlings that had been cultured with distilled water for 6 days and treated with induction medium (100 μM KNO₃ plus 500 μM CaSO₄) for 24h prior to the experiment. The uptake solution was composed of 25-1000 μM KNO₃ and 500 μM CaSO₄. At 25°C, Km and Vmax in the 12 cultivars used in this experiment ranged from 50.9 to 111.7 μM and from 2.24 to 4.99 μmol·g^-1 f.w.·h^-1, respectively. Km obtained in this experiment was roughly coincident with the values demonstrated by earlier studies in some plants including barley. A singnificantly high correlationship (r=0.896) was found between Km and Vmax. Km and Vmax decreased at low temperatures and the extent of the decrease in Km depended on the cultivars. The ratio of Km at 15°C to that at 25d°C ranged from about 1.00 to less than 0.50. Furthermore, it was observed that Vmax was not related to such morphological traits as plant fresh weight, first leaf length, and root length.

収録刊行物

• 日本作物学会紀事

  日本作物学会紀事 61 (2), 251-256, 1992

  日本作物学会

キーワード

• オオムギ
• 温度
• 硝酸イオン吸収
• ミハエリス=メンテンの式
• 幼植物

詳細情報

• CRID: 1390001204284299904
• NII論文ID: 110001733157
• NII書誌ID: AN00189888
• DOI: 10.1626/jcs.61.251
• COI: 1:CAS:528:DyaK38XmsVyltrY%3D
• ISSN: 13490990
• ISSN: 00111848
• NDL書誌ID: 3786950
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010481048
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3786950
• Web Site: http://www.jstage.jst.go.jp/article/jcs1927/61/2/61_2_251/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可