CAM型Mesembyanthemum crystallinum L.のNAD-リンゴ酸脱水素酵素の精製と性質

• 斎藤 和幸

• 縣 和一

• 河原畑 勇

• 山本 優子

• 窪田 文武

書誌事項

タイトル別名

• Purification and Properties of NAD-Dependent Malate Dehydrogenase from Mesembryanthemum crystallinum L. Exhibiting Crassulacean Acid Metabolism.
• Purification and Properties of NAD Depe

抄録

By DEAE-cellulose anion exchange column chromatography, three different forms of NAD-dependent malate dehydrogenase (EC 1.1.1.37) (NAD-MDH) were isolated from leaves of Mesembryanthemum crystallinum L. in the crassulacean acid metabolism mode. NAD-MDH that eluted last from an anion exchange column was purified to a specific activity of 1, 096 units·(mg protein)^-1 using Blue Sepharose CL-6B chromatography. Citrate and adenosine 5'-triphosphate effectively inhibited the activity of NAD-MDH. The inhibition of the enzymatic activity by citrate was reversed by inorganic phosphate and the degree of reversal increased with increasing the concentration of oxaloacetate, the substrate of the reaction. The optimal pH for NAD-MDH activity was around 7.5. Citrate inhibited the enzymatic activity over a wide range of pH and caused a shift in the optimal pH. The enzymatic activity in the presence of citrate was increased by adding inorganic phosphate below pH 7.5. On the basic side, higher than pH 7.5, however, the inhibition by citrate was enhanced by adding inorganic phosphate.

収録刊行物

• 日本作物学会紀事

  日本作物学会紀事 64 (4), 760-766, 1995

  日本作物学会

キーワード

• NAD-リンゴ酸脱水素酵素(EC 1.1.1.37)
• 酵素の精製
• Mesembryanthemum crystallinum L
• ベンケイソウ型酸代謝

詳細情報

• CRID: 1390282679260193920
• NII論文ID: 110001734351
• NII書誌ID: AN00189888
• DOI: 10.1626/jcs.64.760
• ISSN: 13490990
• ISSN: 00111848
• NDL書誌ID: 3912003
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3912003
• Web Site: http://www.jstage.jst.go.jp/article/jcs1927/64/4/64_4_760/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可