Limulus Test Study of Filtrates Obtained by Filter Units.

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  • OHSHIRO SUSUMU
    Department of Hospital Pharmacy, Faculty of Medicine, University of the Ryukyus
  • HOKAMA NOBUO
    Department of Hospital Pharmacy, Faculty of Medicine, University of the Ryukyus
  • FUJIMOTO KATSUYOSHI
    Department of Hospital Pharmacy, Faculty of Medicine, University of the Ryukyus
  • HOBARA NORIO
    Department of Hospital Pharmacy, Faculty of Medicine, University of the Ryukyus

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Other Title
  • フィルターユニットのろ液のリムルステスト反応

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Abstract

The Endotoxin test was applied to detect endotoxin in the filtrates obtained using eight kinds of filter units employed for sterilization. The filtrate used in only one kind of filter unit was positive to this test. The concentrations of endotoxin in all of the filtrates were then determined by Limulus test using an ordinary reagent. In the filtrate that was positive to the endotoxin test, the endotoxin concentration was 0.873 EU/ml. On the other hand, in filtrates from the other filter units, the concentration was below the blank level. When endotoxin was determined in filtrates by Limulus test utilizing an endotoxin-specific reagent, in which a high concentration of endotoxin was detected, one of the five samples indicated a positive level, while the others demonstrated very low or blank level. These results suggest that even if the sample is positive to the endotoxin test, it is necessary to veify with the Limulus test utilizing endotoxin-specific reagent.

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