Genomic in situ hybridization (GISH)によるDiospyros kakiと数種のカキ属植物との類縁関係の推測

• 崔 永娥

• 田尾 龍太郎

• 米森 敬三

• 杉浦 明

書誌事項

タイトル別名

• Genomic in situ Hybridization between Persimmon (Diospyros kaki) and Several Wild Species of Diospyros

抄録

To understand the relationships among the genomes of Diospyros kaki (persimmon) and its wild relatives, we performed genomic in situ hybridization (GISH) on somatic metaphase chromosomes of D. kaki, using digoxigenin (DIG)- 11-UTP labeled DNA of eight Diospyros species. In a series of GISH, it appeared that the D. glandulosa probe hybridized to D. kaki chromosomes resulted with the strongest signal intensity. When comparable GISH was conducted, using unlabeled DNA of D. kaki as a blocking DNA, the highest concentration of blocking DNA was necessary to block hybridization of the D. glandulosa probe. These results suggest that the genomes of D. kaki and D. glandulosa share many common DNA sequences, and that the genome of D. kaki is most closely related to that of D. glandulosa among the species tested.

収録刊行物

• 園芸学会雑誌

  園芸学会雑誌 72 (5), 385-388, 2003

  一般社団法人　園芸学会

キーワード

• Diospyros
• genomic in situ hybridization (GISH)
• persimmon
• repetitive sequences

詳細情報

• CRID: 1390001206303393664
• NII論文ID: 110001803451
• NII書誌ID: AN00025257
• DOI: 10.2503/jjshs.72.385
• COI: 1:CAS:528:DC%2BD3sXns1Wntr8%3D
• ISSN: 1880358X
• ISSN: 00137626
• ISSN: http://id.crossref.org/issn/00137626
• NDL書誌ID: 6702982
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010680942
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6702982
• Web Site: http://www.jstage.jst.go.jp/article/jjshs1925/72/5/72_5_385/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10470353
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • NDL-Digital
  • CiNii Articles
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