パーティクルガン法による除草剤耐性形質転換ユリの作出

• 入船 浩平

• 森本 裕介

• 内浜 正美

書誌事項

タイトル別名

• Production of Herbicide Resistant Transgenic Lily Plants by Particle Bombardment

抄録

Calli induced from bulb scales of Lilium X formolongi were bombarded with the plasmid pAct1-F, which harbors the uidA gene encoding β-glucuronidase (GUS) that is driven by the rice actin-1 promoter. Based on the frequency of GUS-expressing cells in the calli, bombardment conditions were optimized. Calli were bombarded with pDM302 harboring the bar gene, encoding phosphinothricin acetyltransferase (PAT) that is driven by the actin-1 promoter, and selected for bialaphos resistance. Regenerated bialaphos-resistant lily plants were obtained six months after bombardment. PCR analysis suggested that some of the bialaphos-resistant plants were transgenic. Transgenic bialaphos-resistant plants displayed PAT activity and resistance to bialaphos-based herbicide.

収録刊行物

• 園芸学会雑誌

  園芸学会雑誌 72 (6), 511-516, 2003

  一般社団法人　園芸学会

キーワード

• bialaphos
• Lilium × formolongi
• particle bombardment
• transgenic herbicide resistance

詳細情報

• CRID: 1390001206302291840
• NII論文ID: 110001803471
• NII書誌ID: AN00025257
• DOI: 10.2503/jjshs.72.511
• COI: 1:CAS:528:DC%2BD3sXpt1Ohu7Y%3D
• ISSN: 1880358X
• ISSN: 00137626
• NDL書誌ID: 6764057
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010682146
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6764057
• Web Site: http://www.jstage.jst.go.jp/article/jjshs1925/72/6/72_6_511/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10470383
• 本文言語コード: en
• データソース種別:
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  • IRDB
  • NDL
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  • NDL-Digital
  • CiNii Articles
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