Binding of Cd to metallothionein in the placenta of Cd-treated mouse.

• ITOH Norio

• FUJITA Yuki

• NAKANISHI Hirokuni

• KAWAI Yuichi

• MAYUMI Tadanori

• HWANG Gab Soo

• MIN Kyon-son

• ONOSAKA Satomi

• MUTO Norio

• TANAKA Keiichi

Abstract

The toxicological significance of the placental metallothionein (MT) was studied from the viewpoint of cadmium (Cd) intoxication. The Cd concentration was higher in the placenta than the kidneys until 8 hr after a single injection of ^&lt109&gtCdCl₂, but was very low after administration of ^&lt109&gtCd-MT. Compared with lower doses, fetal Cd began to increase at the dose of 2 mg/kg. Alkaline phosphatase activity in the placenta was significantly decreased, and dead fetuses appeared at this dose. The MT concentration was not increased by the Cd injection but the ratio of Cd/Zn in MT increased proportionally to the dose up to 2 mg/kg. MT was detected in the placenta during all stages of pregnancy, and its concentration was higher in the earlier stage. The metal bound to MT was mainly Zn, and very little Cu was bound. In the mice that became pregnant after ^&lt109&gtCd accumulation, redistribution of ^&lt109&gtCd was not observed. MT-I and -II in the placenta were identified by column chromatography and immuno-blotting methods. These findings suggest that the placental MT might play a protective role against Cd toxicity by trapping of the metal.

Journal

• The Journal of Toxicological Sciences

  The Journal of Toxicological Sciences 21 (1), 19-27, 1996

  The Japanese Society of Toxicology

Keywords

• Pregnant mouse

Details

• CRID: 1390282679877812608
• NII Article ID: 110001805779
• NII Book ID: AN00002808
• DOI: 10.2131/jts.21.19
• COI: 1:CAS:528:DyaK28Xis1Wnt7k%3D
• ISSN: 18803989
• ISSN: 03881350
• PubMed: 8852285
• Web Site: http://www.jstage.jst.go.jp/article/jts1976/21/1/21_1_19/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1996122162
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed