エチレン処理されたウンシュウミカン (Citrus unshiu Marc.) 果実の脱緑 : エチレン誘導可溶性クロロフィラーゼ

DOI NDLデジタルコレクション 日本農学文献記事索引 Web Site Web Site 参考文献30件

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タイトル別名
  • Degreening of Citrus unshiu Fruits Via Ethylene-Induced Soluble Chlorophyllase.

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The mechanism of ethylene action on degreening in satsuma mandarin (Citrus unshiu Marc. cv. Nichinan No.1) fruits was investigated using inhibitors of protein synthesis and soluble chlorophyllase (chlorophyll-chlorophyllide hydrolase, Chlase ; E.C. 3.1.1.14). This enzyme which catalyzes the dephytylation of chlorophyll (Chl) to chlorophyllide (Chlide), was partially purified. Chlase catalyzes the first step in the Chl breakdown process. Ethylene treatments promoted the degreening and increased soluble Chlase activity of C. unshiu fruit peels held in the dark. Cycloheximide (CH), an inhibitor of cytoplasmic protein synthesis, inhibited the effects of ethylene, whereas chloramphenicol (CP), an inhibitor of plastid protein synthesis, also weakly inhibited the Chlase activity. These results suggest that ethylene appears to enhance the degreening of the peels through de novo synthesis of Chlase. Moreover, de novo cytoplasmic protein synthesis and chloroplast-dependent enzyme synthesis may regulate Chlase activity in the peel of ethylene-treated C. unshiu fruits. Soluble Chlase was precipitated with acetone from C. unshiu fruit peels and purified by the following method : after ammonium sulfate fractionation, gel-filtration through Sephadex G-25 and Phenyl Sepharose CL-4B (hydrophobic chromatography) were used. The soluble Chlase was purified ca. 203.5-fold with a yield of 45.5%.

収録刊行物

  • 園芸学会雑誌

    園芸学会雑誌 68 (3), 558-562, 1999

    一般社団法人　園芸学会

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