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Abstract
天然無臭ニンニクといわれているネギ属植物について, 植物学的, 生化学的分析を行い, その種の同定を試みた.この植物は, 草丈, 鱗茎ともに通常のニンニクより大きく, 鱗片は花茎に密着して鱗茎を形成する大型のものと, 鱗茎の辺縁に付着する小型のものがあり, これらの数は20に及ぶ.花器はよく発達するが, ニンニクにみられる花序での珠芽形成はない.染色体は2n=32でリーキと同数であり, にんにくの2倍であった.そのDNAの制限断片長多型(RFLP)は, リーキに似ており, ニンニク, エレファントガーリックなどとは異なっていた.アイソザイム分析においてはリーキと最も似たパターンを示した.鱗茎細胞内でのアリイナーゼ(C-Sリアーゼ)mRNAの発現は, 他種に比べ少なかったが, 大きさは等しく, 1.9 kbであった.この植物のアリイナーゼのN末端25アミノ酸残基の配列はリーキのそれと一致し, ニンニクとは2残基相違した.以上の結果から, この植物は, リーキと極めて近縁の植物であり, Allium ampeloprasum L.の中に分類することができると考えられる.
We tried to identify the unidentified Allium plant, 'Mushuu-ninniku', which resembles garlic (A. sativum L.) but has no garlic odor. 'Mushuu-ninniku' is much larger than a garlic. Its plant height and bulb weight are twice that of garlic. It develops many different sized cloves, inflorescences, and flowers, but no inflorescent bulbil. The chromosome number of 'Mushuu-ninniku' is 2n=32, similar to leek (A. ampeloprasum L.), but double that of garlic. In restriction fragment length polymorphisms (RFLPs), 'Mushuu-ninniku' produced patterns similar to those of leek and quite unlike those of great-headed garlic (A. ampeloprasum L.), which usually has a prominent bulb similar to 'Mushuu-ninniku'. Isozyme analyses also showed some similarity between 'Mushuu-ninniku' and leek. Although the alliinase mRNA size (1.9 kb) of 'Mushuu-ninniku' was identical to those of other Allium plants examined, the N-terminal 25 amino acids sequence of 'Mushuu-ninniku' alliinase was different from those of other Allium plants examined except leek. From these results, we concluded that 'Mushuu-ninniku' belongs to Allium ampeloprasum.
Journal
- Journal of the Japanese Society for Horticultural Science [List of Volumes]
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Journal of the Japanese Society for Horticultural Science 71(3), 362-369, 2002-05-15 [Table of Contents]
The Japanese Society for Horticultural Science