電気融合によるネギ (Allium fistulosum L.) とタマネギ (A. cepa L.) との体細胞雑種の育成

DOI NDLデジタルコレクション 日本農学文献記事索引 Web Site Web Site 参考文献30件

書誌事項

タイトル別名
  • Production of Somatic Hybrid Plants between Japanese Bunching Onion(Allium fistulosum L.) and Bulb Onion(A. cepa L.) via Electrofusion.

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抄録

Interspecific somatic hybrids between Japanese bunching onion (Allium fistulosum L., 2n=2x=16) and bulb onion (A. cepa L., 2n=2x=16) were produced by the electorofusion of protoplasts. A concentration of iodoacetamide (IOA), suitable for inhibiting the formation of colonies from protoplasts in Japanese bunching onion, was used. IOA-treated protoplasts of Japanese bunching onion and untreated protoplasts of bulb onion were mixed together and fused, with the expectation that only heterokaryons would selectively develop into colonies. Four hundred and seventeen colonies had formed from fusion products after a 45- day culture, of which approximately 80% developed into calli. Plant regeneration was achieved in 33 out of 325 (10.1%) calli. Some regenerants expressed abnormalities, but two were successfully transplanted in a greenhouse. Cytogenetical and DNA analyses revealed these two regenerants to be amphidiploids (2n=4x=32). Furthermore, it was shown that another three regenerants possessed the nuclear genome of Japanese bunching onion, whereas, their chloroplasts were from bulb onion. The application of these hybrids to a breeding program for Japanese bunching onion is discussed.

収録刊行物

  • 園芸学会雑誌

    園芸学会雑誌 71 (5), 623-631, 2002

    一般社団法人　園芸学会

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