神経芽細胞腫に対するSynchronization療法の研究 内藤 春彦 佐々木 文章 秦 温信 内野 純一 葛西 洋一 特定非営利活動法人日本小児外科学会 日本小児外科学会雑誌 0288609X 18 1 141 146 1982-02-20 The synchronizing effect of the combination chemotherapy of vincristine (VCR) and cyclop-hosphamide (CPM) on C-1300 murine neuroblastoma was investigated in vivo. A synchronizing effect of intraperitoneally administered (i.p.) VCR was observed by flowcytometry using Fried's formula for analysis and autoradiography. VCR (0.1 mg/kg) i.p. induced a G_2+M phase accumulation after 6 hours and an S-phase accumulation 9 to 18 hours later. The combined treatment with VCR (0.1 mg/kg) followed by CPM (100 mg/kg) after 12 hours weekly caused remarkable inhibition of the tumor growth during 4 weeks. The improvement of the animal survival rates were not to good as the tumor growth inhibitory effect. This may be because of the timing of injection of CPM, which may induce an S-phase accumulation of normal bone marrow cells and increased animal toxicity. To use a synchroniation method in vivo, it is important to know the cell cycles of both tumor and normal cells. 1982-02-20 110002095802 AN00192281 2483486 JPN NDL NII-ELS 1 Synchronization Therapy for C-1300 Murine Neuroblastoma Naito Haruhiko Sasaki Fumiaki Hata Yoshinobu Uchino Junichi Kasai Yoichi The Japanese Society of Pediatric Surgeons (JSPS) Journal of the Japanese Society of Pediatric Surgeons The synchronizing effect of the combination chemotherapy of vincristine (VCR) and cyclop-hosphamide (CPM) on C-1300 murine neuroblastoma was investigated in vivo. A synchronizing effect of intraperitoneally administered (i.p.) VCR was observed by flowcytometry using Fried's formula for analysis and autoradiography. VCR (0.1 mg/kg) i.p. induced a G_2+M phase accumulation after 6 hours and an S-phase accumulation 9 to 18 hours later. The combined treatment with VCR (0.1 mg/kg) followed by CPM (100 mg/kg) after 12 hours weekly caused remarkable inhibition of the tumor growth during 4 weeks. The improvement of the animal survival rates were not to good as the tumor growth inhibitory effect. This may be because of the timing of injection of CPM, which may induce an S-phase accumulation of normal bone marrow cells and increased animal toxicity. To use a synchroniation method in vivo, it is important to know the cell cycles of both tumor and normal cells. 内藤 春彦 Naito Haruhiko 北海道大学第一外科 First Department of Surgery, Hokkaido University School of Medicine 佐々木 文章 Sasaki Fumiaki 北海道大学第一外科 First Department of Surgery, Hokkaido University School of Medicine 秦 温信 Hata Yoshinobu 北海道大学第一外科 First Department of Surgery, Hokkaido University School of Medicine 内野 純一 Uchino Junichi 北海道大学第一外科 First Department of Surgery, Hokkaido University School of Medicine 葛西 洋一 Kasai Yoichi 北海道大学第一外科 First Department of Surgery, Hokkaido University School of Medicine