Inactivation of Ribonuclease A in Aqueous Solution by Ionizing Radiation
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Ribonuclease A were gamma-irradiated in a dilute aqueous solution. The G° value of inactivation of the enzyme was 0.45. When the enzyme lost its enzymatic activity significantly, some physicochemical or chemical properties were found to be altered, while others were not. Unaltered properties were ‘type’ of amino acid terminals, optimum pH and pH-enzyme activity curves. Properties affected by irradiation were a destruction of some amino acid residues, an alteration of electrophoretic mobility, and an incresed reactivity to diazo-1-H-tetrazole. The extents of alteration of the latter two properties were dependent upon the radiation dose. These results suggest that the ionizing radiations induce a local conformational change in the enzyme molecule and that this may play an important role in the inactivation of the enzymatic activity of ribonuclease A.
収録刊行物
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- Journal of Radiation Research
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Journal of Radiation Research 13 (4), 221-229, 1972
Journal of Radiation Research 編集委員会
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詳細情報 詳細情報について
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- CRID
- 1390001205217575040
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- NII論文ID
- 110002323065
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- NII書誌ID
- AA00705792
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- COI
- 1:CAS:528:DyaE3sXjvF2lsQ%3D%3D
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- ISSN
- 13499157
- 04493060
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- PubMed
- 4675921
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- PubMed
- NDL-Digital
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可