Monochromatic X-ray irradiation system (0.08-0.4nm) for radiation biology studies using synchrotron radiation at the Photon Factory.

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  • 小林 克己
    高エネルギー物理学研究所放射光実験施設
  • HIEDA KOTARO
    Biophysics Laboratory, Department of Physics, Rikkyo University
  • MAEZAWA HIROSHI
    Department of Radiology, School of Medicine, Tokai University
  • ANDO MASAMI
    Photon Factory, National Laboratory for High Energy Physics
  • ITO TAKASHI
    Institute of Physics, College of Arts and Sciences, University of Tokyo

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A monochromatic X-ray irradiation system in the 0.08-0.4 nm wavelength range is described. This uses for the first time, synchrotron radiation at the Photon Factory as an X-ray source (2.5 GeV electron storage ring). It consists of a beam shutter, a Si crystal monochromator, ionization chambers for monitoring exposures, a sample stage, and a control system. The beam is 2.7 × 30 mm at the sample position with a fairly uniform intensity of several kR/min (one C · kg-1 · min-1). The wavelength is an extremely pure Δλ/λ ?? 10-3. A 35 mm culture dish is moved across the vertical height (2.7 mm) of the X-ray beam using a sample scanning stage. This improves the uniformity of the radiation intensity on the sample. The sample can be irradiated under normal or controlled atmospheric conditions. Since 1984, a variety of biological materials have been irradiated, including cultured mammalian cells. Results of yeast cells are presented to illustrate the performance of this irradiation system.

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