A new automated method to analyze urinary 8-hydroxydeoxyguanosine by a high-performance liquid chromatography-electrochemical detection system
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- KASAI HIROSHI
- Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health
書誌事項
- タイトル別名
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- A New Automated Method to Analyze Urinary 8-hydroxydeoxyguanosine by a High-performance Liquid Chromatography-Electrochemical Detector System.
- New Automated Method to Analyze Urinary 8 Hydroxydeoxyguanosine by a High performance Liquid Chromatography Electrochemical Detector System
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抄録
A new method was developed to analyze urinary 8-hydroxydeoxyguanosine (8-OH-dG) by high-performance liquid chromatography (HPLC) coupled to an electrochemical detector (ECD). This method is unique because (i) urine is first fractionated by anion exchange chromatography (polystyrene-type resin with quaternary ammonium group, sulfate form) before analysis by reverse phase chromatography; and (ii) the 8-OH-dG fraction in the first HPLC is precisely and automatically collected based on the added ribonucleoside 8-hydroxyguanosine marker peak, which elutes 4-5 min earlier. Up to 1,000 human urine samples can be continuously analyzed with high accuracy within a few months. This method will be useful for studies in radiotherapy, molecular epidemiology, risk assessment, and health promotion.<br>
収録刊行物
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- Journal of Radiation Research
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Journal of Radiation Research 44 (2), 185-189, 2003
Journal of Radiation Research 編集委員会
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詳細情報 詳細情報について
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- CRID
- 1390282680191769472
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- NII論文ID
- 110002328883
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- NII書誌ID
- AA00705792
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- COI
- 1:CAS:528:DC%2BD3sXmslCqurw%3D
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- ISSN
- 13499157
- 04493060
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- NDL書誌ID
- 6610410
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- PubMed
- 13678349
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- NDL-Digital
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可