生理浸透圧凍害防御剤の凍結解凍過程における細胞への影響とElectroporationによる細胞内導入の効果 : Sp2細胞に対するアルギン酸,トレハロース水溶液について(熱工学,内燃機関,動力など)  [in Japanese] Effects of Physiological Isotonic Cryoprotectants on Living Cells during the Freezing-Thawing Process and Effects of their uptake by Electroporation : Alginate-Trehalose Solutions to Sp2 Cells  [in Japanese]

In the present study, effects of iso-osmolar alginate and trehalose solutions on electroporated or unpulsed control murine myeloma cells (Sp2) during the freezing-thawing process are discussed. To investigate the effects of each cryoprotectant, the freezing-thawing process of electroporated or intact cells were observed, which were suspended in alginate-NaCl solution, alginate-trehalose solution or trehalose solution. The microscopic observation took place also at various cooling rates (-1℃/min, -90℃/min, -5℃/min). It was found that extracellular ice grains in the solution containing trehalose did not mechanically suppress cells even at lowest cooling rate. Further, only a seldom intracellular ice nucleation was observed at all cooling rate for the pulsed or unpulsed cells which were suspended in trehalose solution.