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It is necessary to develop a small positive pressure control system for the closed ecology experiment facilities because of the needs to prevent contamination from the atmosphere and to protect against over differential pressure loading. In the present study, a numerical analysis method was developed to calculate the quantity of state of the closed module which is fitted with rubber buffers for the small positive pressure control system. Experiments to examine the pressure change of the closed module caused by rapid temperature control of an air conditioner were carried out at Biosphere-J. Comparison of calculated and experimental results showed that the present dynamic simulation is suited to estimating the quantity of state of the closed module.