The Biological Activity and Chemical Extraction and Quantitative Determination of Histamine and Serotonin Produced by Anaphylaxis in vitro

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  • In vitroのアナフィラキシー性反応の際生起するヒスタミン及びセロトニンの生物学的活性値と化学的抽出並びに定量
  • In vitro ノ アナフィラキシーセイ ハンノウ ノ サイ セイキ スル ヒスタミン オヨビ セロトニン ノ セイブツガクテキ カッセイチ ト カガクテキ チュウシュツ ナラビニ テイリョウ

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Abstract

Guinea-pigs were passively sensitized with anti-egg-albumin rabbit serum. Twenty hours after the sensitization they were killed by heart puncture and the lung, small-intestine and liver were extracted and suspended in Tyrode solution at 37℃. These materials were divided into two groups; one was control and the other was for anaphylaxis in vitro. Two percent of egg-albumin was added into the latter groups and shocked in vitro. The biological activity and chemical components of the supernatants by centrifugation were examined. As the chemical components appeared in the medium during anaphylaxis in vitro histamine and serotonin were mainly tested. Histamine extraction was followed by Dwetch and Loew's method (1954) by cotton acid succinate and determined quantitatively by the biological technique, and serotonin was extracted by modified method described by Udenfriend and Weissbach et al (1955) and quantitatively determined by ultraviolet absorption. The solutions obtained during anaphylaxis in vitro showed more severe contracting action of the guinea pig's intestine than the control solutions. However the results obtained with the antihistamine and antiacetylcholine drug suggested that histamine, acetylcholine and serotonin might be produced during anaphylaxis in vitro. Namely, histamine in the lung's solution during anaphylaxis in vitro was proved to be increased about 2 times and serotonin was 3 times than the control solution. Histamine in the liver's solution during anaphylaxis in vitro was also found to be increased about 50% and serotonin about 2 times. Serotonin value in the case of the small intestine was about 50% higher than the control solution, but not a remarkble change was recognized in histamine value. The findings that histamine and serotonin might be increased during anaphylaxis in vitro were clearly demonstrated by the chemical analysis.

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