真菌の免疫学的活性物質の研究 : 3.Sporotrichum, CryptococcnsおよびAspergil菌体抽出抗原の皮内反応活性および数種の真菌沈降抗原の構成糖について  [in Japanese] Studies on the Immunologically Active Substances in Fungi : 3.The chemical properties of skin test antigens from organisms of Sporotrichum Schenckii, Cryptococcus neoformans and Aspergillus fumigatus, and of serologically active components in several mycotic antigens  [in Japanese]

Skin test activities of antigens separated from organisms of Sporotrichum Schenckii and Cryptococcus neoformans by French press extraction and of and antigen obtained from Aspergillus fumigatus by phenol extraction were reduced by the treatment with proteolytic enzyme, papain or pronase. From the results obtained in this and in two previous papers, it was concluded that the active principle of skin test antigen in mycotic infections was protein. On the other hand, the main component responsible for precipitation reaction was polysaccharide. In order to determine the chemical property of active component for precipitation reaction found in antigens from 6 species of fungi, sugar components in antigen-antibody complexes were analyzed by gas liquid chromatography. Only mannose was detected in Ag-Ab complexes of Candida antigen and histoplasmin, while the Candida and histoplasmin polysaccharide contained mannose and a small amount of glucose. In the same manner, xylose and mannose were detected in Cryptococcus Ag-Ab complex, rhamnose and mannose in Sporotrichum and galactose and mannose in Aspergillus, respectively. These antigens also contained a small amount of glucose, but no glucose was detected in these Ag-Ab complexes. From these results, it was concluded that the active component for precipitation reaction was mannan in Candida and histoplasmin polysaccharide, xylomannan in Cryptococcus, rhamnomannan in Sporotrichum and galactomannan in Aspergillus, respectively.