^<131>I標識室内塵による抗室内塵抗体の検索 : その基礎的研究  [in Japanese] Detection of Anti House Dust Antibody by Use of ^<131> I labeled House Dust. : Experimental Studies.  [in Japanese]

1) Anti house dust sera were prepared by injecting the concentrated extract of house dust into three rabbits. Two sera out of three formed three precipitin bands against the same extract in double diffusion test. Another serum formed four bands against the extract, but produced only two bands against another lot of house dust. These two bands fused with two of four bands formed by the house dust used immunization. 2) The main component of the house dust extract migrated more slowly than human serum albumin or egg albumin through Sephadex G 200 column. 3) When the mixture of house dust extract and ^<131>I labeled house dust was filtrated through Sephdex G 200 column, the peak of redioactivity appeared before the peak of optical density at 280 mμ. 4) By redioimmunoelectrophoresis, it was shown that ^<131>I labeled house dust reacted with IgG of rabbit. However, there were nonspecfic reaction of ^<131>I labeled house dust with albumin and α-globulin of both immunized and normal rabbit sera. The use of anti rabbit Fab instead of anti rabbit serum succeeded in clearly demonstrating only specific reaction of ^<131>I labeled house dust with immunoglobulins. 5) Invisible complex of ^<131>I labeled house dust and anti house dust antibody was also detected by radioautography.