72. イネGID1はジベレリンに対する核内受容体である

• 中嶋 正敏

  東大院・農生科応生化

• 田中 美弥子

  名大・生物機能開発利用研究センター

• 芦刈 基行

  名大・生物機能開発利用研究センター

• 伊藤 博紀

  名大・生物機能開発利用研究センター

• 加藤 悦子

  (独)農業生物資源研究所

• 小林 正智

  理研・バイオリソースセンター

• 北野 英己

  名大・生物機能開発利用研究センター

• 松岡 信

  名大・生物機能開発利用研究センター

• 山口 五十麿

  東大院・農生科応生化:東大・生物生産工学研究センター

書誌事項

タイトル別名

• 72. Identification of rice cytosolic gibberellin-receptor GID1

抄録

Gibberellins (GAs) regulate various developmental processes in plants. Recently we identified a rice Gibberellin Insensitive Dwarf1 (GID1) gene as for an ingredient of GA-insensitive dwarf mutants by positional cloning and detected the GA-binding activity in its recombinant protein (rGID1) prepared using an E. coli expressional system. We studied its ligand-selectivity using some GAs and their derivatives, which made it clear that the affinities to the rGID1 and their physiological activities are almost parallel and the highest affinity (K_d value) to it was shown to be around 10^<-7>M with GA_4. Three mutated rGID1s responsible for sever dwarf phenotype lost their GA-binding activity. We also checked the in vivo interaction between the GID1 and the negative regulator of GA-signaling, SLR1, using a yeast two-hybrid system. Their GA-dependent interaction was clearly confirmed. Taken together with other informations : (i) endogenous GA carrying physiologically active form accumulated 100-fold more in the mutants compared with that in a wild-type plant, (ii) overexpression of the gene leads to GA-hypersensitive, and (iii) the GID1-GFP was preferentially localized in nuclei, we concluded that the GID1 is a cytosolic GA receptor which localizes and functions in nuclei.

収録刊行物

• 植物化学調節学会 研究発表記録集

  植物化学調節学会 研究発表記録集 40 (0), 91-, 2005

  一般社団法人 植物化学調節学会

詳細情報

• CRID: 1390282679400840448
• NII論文ID: 110002552770
• DOI: 10.18978/jscrpanb.40.0_91
• ISSN: 21896313
• ISSN: 09191887
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可