Regulation of Bradykinin-Stimulated Cation Entry Into Endothelial Cells by Tyrosine Kinase
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- Takahashi Reiko
- Department of Internal Medicine III
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- Watanabe Hiroshi
- Department of Internal Medicine III
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- Kakizawa Hiroyasu
- Department of Internal Medicine III
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- Zhang Xu-Xia
- Department of Internal Medicine III
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- Hayashi Hideharu
- Photon Medical Research Center, Hamamatsu University School of Medicine
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- Ohno Ryuzo
- Department of Internal Medicine III
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抄録
In endothelial cells, bradykinin stimulates the release of intracellular Ca2+, which is followed by the entry of extracellular Ca2+ into the cells. However, the mechanism underlying this Ca2+ entry is not well understood. To investigate the possible implication of tyrosine kinases in bradykinin-mediated Ca2+ signaling in endothelial cells, cultured porcine aortic endothelial cells were loaded with fura-2/AM, and Mn2+ influx into the cells was determined by the quenching of fluorescence intensity of fura-2 at 360 nm excitation. The tyrosine kinase inhibitors genistein and herbimycin A attenuated not only Ca2+ influx but also Mn2+ influx from the extracellular space without affecting the release of Ca2+ from internal stores in bradykinin-treated cells. In contrast to tyrosine kinase inhibitors, the tyrosine phosphatase inhibitor vanadate stimulated Ca2+ influx as well as Mn2+ influx. On the other hand, both an inactive analog of genistein, daidzein, and an inhibitor of diacylglycerol (DAG) kinase, ethylene glycol dioctanoate, were without effect on [Ca2+]i following the stimulation of agonist. These findings suggest that tyrosine kinase is involved in the regulation of cation influx in endothelial cells. (Jpn Circ J 1997; 61: 1030 - 1036)
収録刊行物
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- JAPANESE CIRCULATION JOURNAL
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JAPANESE CIRCULATION JOURNAL 61 (12), 1030-1036, 1997
社団法人 日本循環器学会
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詳細情報 詳細情報について
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- CRID
- 1390001206428309248
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- NII論文ID
- 110002564471
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- NII書誌ID
- AA00690731
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- COI
- 1:CAS:528:DyaK1cXosVOrsA%3D%3D
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- ISSN
- 13474839
- 00471828
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- PubMed
- 9412868
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可