Pyrophosphorylase Activities towards Nucleoside Diphosphate Sugars in Bifidobacterium bifidum : Biochemical studies on Bifidobacterium bifidum(I) :

• TOCHIKURA,TATSUROKURO

  Department of Food Science and Technology, Kyoto University

• LEE,LYANG JA

  Department of Food Science and Technology, Kyoto University

• KIMURA,AKIRA

  Department of Food Science and Technology, Kyoto University

抄録

The cell-free extract of Bifidobacterium bifidum grown on glucose, lactose, or galactose as a carbon source showed relatively high pyrophosphorylase activities towatds uridine diphosphate glucose (UDP-glucose) and uridine diphosphate galactose(UDP-galactose) and a slight activity towards uridine diphosphate xylose(UDP-xylose). No activity was found towards other sugar nucleotides.Various strains of lactic acid bacteria and actinomycetes showed little or no activities of UDP-glucose and UDP-galactose pyrophosphorylase.The activities of galactokinase, galactose-1-phosphate uridyltransferase and UDP-galactose 4-epimerasem, which are involved in the Leloir pathway, and of related nucleotide kinases, were also investigated. Activities of Leloir pathway enzymes were found in not only galactose- and lactose-grown cells but also glucose-grown cells of B. bifidum.The enzymes were separated by column chromatography on DAEA-cellulose.The activity of UCP-galactose pyrophosphorylase was one-third that of UDP-glucose pyrophosphorylase even in the DEAE-cellulose fraction free of UDP-galactose 4-epimerase and galactose 1-phosphate uridyltransferse. The existence of an additional pathway of galactose metabolism was also discussed.

収録刊行物

• Journal of fermentation technology

  Journal of fermentation technology 55 (2), 122-129, 1977-04-25

  Society of Fermentation Technology, Japan

詳細情報

• CRID: 1543387470136225664
• NII論文ID: 110002672392
• NII書誌ID: AA00698096
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/11076672
• 本文言語コード: en
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  • CiNii Articles