Induction of Cellulases and Hemicellulases by Tamarind (Tamarindus indica) Kernel Polysaccharide :

  • GHOSH,B.S.
    Indian Jute Industries' Research Association (IJIRA)
  • KUNDU,A.B.
    Indian Jute Industries' Research Associatin (IJIRA)

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Tamarind kernel polysaccharide (TKP) which is available i India in abundance could be used as an excellent substrate for production of cellulases, hemicellulases, β-glucosidase and β-xylosidase. A growth medium of a known cellulolytic strain, Aspergillus terreus containing TKP has been optimised to get enhanced yields of cellulase and hemicellulase for practical application in jute manufacturing units around Calcutta.Of the large number of sugars and treated and untreated cellulosics tested, TKP was found to produce the highest amounts of cellulases, hemicellulases, β-glucosidase, β-xylosidase and extra-cellular protein. The effects of the use of different inorganic nitrogenous substances, nutrients and surfactants in the optimized medium have been tested. Both initial pH of the medium and final pH of the culture filtrate have been found to have a marked effect on enzyme production, especially β-glucosidase production.

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