Controlled-Batch Fermentations of Escherichia coli at Low Ammonium Concentrations :

• KOLE,M.M.

  Biotechnology Department, Alberta Research Council

• THOMPSON,B.G.

  Biotechnology Department, Alberta Research Council

• GERSON,DONALD F.

  Biotechnology Department, Alberta Research Council

Abstract

Ammonium-controlled fed-batch fermentations of Escherichia coli were studied at different constant ammonium concentrations from 0.5 mM to 5.0 mM. The ammonia electrode (Orion 95-12) used to measure and control ammonium concentration was stable throughout the fermentation period. The growth yields for ammonium and glucose increased approximately 20-fold as the ammonium concentration increased from 0.5 mM to 2.5 mM. At constant, low ammonium concentrations growth rate, biomass production, glucose utilization, protein production, and ion utilization were all suboptimal, indicating that the minimum ammonium concentration necessary for normal metabolism in E. coli is approximately 5 mM.

Journal

• Journal of fermentation technology

  Journal of fermentation technology 63 (2), 211-214, 1985-04-25

  Society of Fermentation Technology, Japan

Details

• CRID: 1542261570229491072
• NII Article ID: 110002672923
• NII Book ID: AA00698096
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/11077560
• Text Lang: en
• Data Source:
  • NDL-Digital
  • CiNii Articles