Molecular Cloning and Nucleotide Sequence of the Pectate Lyase Gene from<i>Pseudomonas marginalis</i>N6301

• Nikaidou Naoki

• Kamio Yoshiyuki

• Izaki Kazuo

書誌事項

タイトル別名

• Molecular Cloning and Nucleotide Sequence of the Pectate Lyase Gene from Pseudomonas marginalis N6301.

抄録

Pectate lyase was purified approximately 29-fold to electrophoretic homogeneity from Pseudomonas marginalis N6301. A pectate lyase (PL; EC4.2.2.2) gene of the strain was cloned and expressed in Escherichia coli. The nucleotides of the PL gene (pel) were sequenced. An open reading frame that encodes a polypeptide (molecular weight: 40, 812) composed of 380 amino acids including a 29 amino acid signal peptide was assigned. The structural gene of pel consisted of 1140 base pairs. The nucleotide sequence of the 5'-flanking region of pel showed a consensus sequence of the promoter region of the pectin lyase gene (pnl) in P. marginalis N6301, a Pribnow box, and a ribosome binding site as found in E. coli.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 57 (6), 957-960, 1993

  公益社団法人 日本農芸化学会

キーワード

• Organic Chemistry
• Molecular Biology
• Applied Microbiology and Biotechnology
• General Medicine
• Biochemistry
• Analytical Chemistry
• Biotechnology

詳細情報

• CRID: 1390001206470416256
• NII論文ID: 110002676387
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.57.957
• COI: 1:CAS:528:DyaK3sXlvFKqtL4%3D
• ISSN: 13476947
• ISSN: 09168451
• PubMed: 7763884
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.57.957
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可