Binding of Saccharides to Abrin-b Isolated from<i>Abrus precatorius</i>Seeds

• Ohba Hideki

• Yamasaki Nobuyuki

• Eguchi Koichirou

• Funatsu Gunki

書誌事項

タイトル別名

• Binding of Saccharides to Abrin-b Isolated from Abrus precatorius Seeds.

抄録

The nature of the binding of saccharides to arbin-b, a toxic lectin isolated from Abrus precatorius seeds, was studied by equilibrium dialysis and fluorescence spectroscopy. Equilibrium dialysis data indicate that abrin-b has two saccharide-binding sites, a high affinity site (HA-site) and a low affinity site (LA-site), to which both galactopyranosides and N-acetylgalactosamine can bind. With excitation at 290 nm, abrin-b displayed a fluorescence spectrum with an emission maximum at 345nm. Upon binding with specific saccharides, this spectrum shifted to a wavelength shorter by 5 nm, suggesting that saccharides bind to abrin-b in such a manner as to induce a change in the environment of the tryptophan residue or residues at or near the respective binding sites. From the variation of fluorescence at 320 nm with saccharide concentrations, the association constants for binding of saccharides to the respective sites were measured. The results suggest that the HA-site has a subsite favorable for saccharides having β-1, 4 linked galactopyranoside at the non-reducing end like lactose in addition to the galactose-recognition site, while the LA-site may not have such a subsite.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 57 (9), 1409-1413, 1993

  公益社団法人 日本農芸化学会

キーワード

• Organic Chemistry
• Molecular Biology
• Applied Microbiology and Biotechnology
• General Medicine
• Biochemistry
• Analytical Chemistry
• Biotechnology

詳細情報

• CRID: 1390001206471752064
• NII論文ID: 110002676523
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.57.1409
• ISSN: 13476947
• ISSN: 09168451
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.57.1409
• 本文言語コード: en
• データソース種別:
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  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可