Isolation and Characterization of a Trehalulose-producing Strain of<i>Agrobacterium</i>

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  • Isolation and Characterization of a Trehalulose-producing Strain of Agrobaeterium.

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A novel strain, MX-232, which produced much trehalulose (1-O-α-D-glucopyranosyl-D-fructose), was isolated from a soil sample in Udonthani, Thailand. The isolate was a Gram-negative aerobic rod, motile with peritrichous flagella, and had a high ability to convert sucrose into trehalulose and isomaltulose (palatinose, 6-O-α-D-glucopyranosyl-D-fructose) with α-glucosyltransferase. The results of physiological characterization, G+C content, and pathogenicity tests on several plants showed that MX-232 is a strain of Agrobacterium radiobacter. When free cells and 20% (w/v) sucrose solution were used in the reaction, the yield of trehalulose was 88-90% (w/w). And with immobilized cells, the yield was about 85% when 40-50% (w/w) sucrose solution was used as the substrate. Using immobilized cells in a column reaction was good for producing trehalulose. This report suggests MX-232 has the highest ability to produce trehalulose from sucrose.

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