Opimization of Conditions for Production of Uridine by a Mutant of<i>Bacillus subtilis</i>

• Doi Muneharu

• Tsunemi Yutaka

• Asahi Satoru

書誌事項

タイトル別名

• Microbial Production of Uridine. Part IV. Opimization of Conditions for Production of Uridine by a Mutant of Bacillus subtilis.

抄録

This work was designed to examine culture variables influencing the production of uridine by the Bacillus subtilis mutant No. 556. A combination of corn steep liquor (4%) + corn gluten meal (0.7%) + urea (2%) was the most suitable nitrogen source for the production of uridine ; a high level of ammonium ions in the medium brought about abundant cell growth in the early stage of incubation and was detrimental to the fermentation. The addition of calcium salts, such as C_aCO₃ and C_aCl₂, was also essential for obtaining high yields of uridine. Among the carbon sources tested, glucose gave the best result. The optimum temperature and oxygen transfer rate for producing uridine were around 38°C and 20 x 10^-7 molO₂ / min / ml, respectively. Under optimum conditions in a 6000-liter fermentor, strain No. 556 produced 65mg/ml of uridine from 18% glucose.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 58 (9), 1608-1612, 1994

  公益社団法人 日本農芸化学会

キーワード

• Organic Chemistry
• Molecular Biology
• Applied Microbiology and Biotechnology
• General Medicine
• Biochemistry
• Analytical Chemistry
• Biotechnology

詳細情報

• CRID: 1390282681446978304
• NII論文ID: 110002677159
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.58.1608
• ISSN: 13476947
• ISSN: 09168451
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.58.1608
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可