Characterization of α-Glucosyltransferase from<i>Pseudomonas mesoacidophila</i>MX-45

• Nagai Yukie

• Sugitani Toshiaki

• Tsuyuki Ken-ichirou

書誌事項

タイトル別名

• Characterization of .ALPHA.-Glucosyltransferase from Pseudomonas mesoacidophila MX-45.

抄録

α-Glucosyltransferase was purified from Pseudomonas mesoacidophila MX-45. The molecular weight was estimated to be 63, 000 by SDS-PAGE, and the isoelectric point was pI 5.4. For enzyme activity based on sucrose decomposition, the optimum pH and the optimum temperature were pH 5.8 and 40°C, respectively. The ranges of stable pH and temperature were pH 5.1-6.7 and below 40°C, respectively. The purified enzyme of MX-45 converted sucrose into trehalulose (1-O-α-D-glucopyranosyl-D-fructose) and isomaltulose (palatinose, 6-O-α-D-glucopyranosyl-D-fructose) simultaneously, and the ratio of trehalulose to isomaltulose increased at lower reaction temperatures. Therefore, optimum conditions for trehalulose production were pH 5.5-6.5 at 20°C. The yield of trehalulose from sucrose (20-40% solution) was 91%. The K_m for sucrose was 19.2±3.3 mM estimated by the Hanes-Woolf plot. Product inhibition was observed, and the product inhibition constant was 0.17M. Hg²⁺, Fe³⁺, Cu²⁺, Mg²⁺, Ag⁺, Pb²⁺, glucono-1, 5-lactone, and Tris (hydroxymethyl) aminomethane inhibited the reaction.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 58 (10), 1789-1793, 1994

  公益社団法人 日本農芸化学会

キーワード

• Organic Chemistry
• Molecular Biology
• Applied Microbiology and Biotechnology
• General Medicine
• Biochemistry
• Analytical Chemistry
• Biotechnology

詳細情報

• CRID: 1390282681453331968
• NII論文ID: 110002677207
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.58.1789
• COI: 1:CAS:528:DyaK2MXitVCnt7s%3D
• ISSN: 13476947
• ISSN: 09168451
• PubMed: 7765505
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.58.1789
• 本文言語コード: en
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