Purification and Characterization of<i>α</i>-Keto Ester Reductases from Bakers’ Yeast

• Nakamura Kaoru

• Kondo Shin-Ichi

• Kawai Yasushi

• Nakajima Nobuyoshi

• Ohno Atsuyoshi

書誌事項

タイトル別名

• Purification and Characterization of .ALPHA.-Keto Ester Reductases from Bakers' Yeast.
• Purification and characterization of α-keto ester reductases from bakers' yeast

抄録

Three α-keto ester reductases (yeast keto ester reductase, YKER-II, -IV, -V) were purified from bakers'yeast. YKER-II, -IV, and -V are dimeric, monomeric, and dimeric enzymes, respectively, and molecular masses are estimated to be 58, 31-39, and 83kDa, respectively, based on gel filtration and SDS-polyacrylamide electrophoresis. Kinetic parameters and stereoselectivities in reduction of α-keto esters have been measured. YKER-IV contributes mainly to reduction by bakers' yeast at low substrate concentrations, and is useful for synthetic purposes.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 58 (12), 2236-2240, 1994

  公益社団法人 日本農芸化学会

キーワード

• Organic Chemistry
• Molecular Biology
• Applied Microbiology and Biotechnology
• General Medicine
• Biochemistry
• Analytical Chemistry
• Biotechnology

詳細情報

• CRID: 1390001206474156672
• NII論文ID: 110002677326
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.58.2236
• COI: 1:CAS:528:DyaK2MXjtFWqsr0%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.58.2236
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可