Role of the Carbohydrate Moiety of a Glucoamylase from<i>Aspergillus awamori</i>var.<i>kawachi</i>in the Digestion of Raw Starch

DOI Web Site PubMed 被引用文献8件 参考文献23件

書誌事項

タイトル別名
  • Role of the Carbohydrate Moiety of a Glucoamylase from Aspergillus awamori var. kawachi in the Digestion of Raw Starch.

この論文をさがす

抄録

The digestion of raw starch by a glucoamylase (GA MU-H) from a mutant strain of Aspergillus awamori var. kawachi was closely correlated with mannoside chains O-linked to the Gp-I region (A470-V514), but not sugar chains N-linked to catalytic GAI' domain of GA MU-H. The partial replacement of mannose residues by glucose residues led to a significant decrease raw starch digestion. By the substitution of D20 for H20 in the reaction mixture, the raw starch digestion of GA MU-H decreased to 80% of that at 30°C, although the rate of hydrolysis of soluble starch by and the ability to bind β-cyclodextrin of GA MU-H were unchanged. Glycerol, known as an antichaotropic reagent, decreased the raw starch digestion of GA MU-H significantly. However, it did not have any effect on the enzymatic activity for soluble starch when soluble starch was the substrate. The efficient digestion of raw starch with raw starch-digesting glucoamylase needed the mannoside chains O-linked to the Gp-I region, which were suggested to contribute to digestion of raw starch through the interaction with water.

収録刊行物

被引用文献 (8)*注記

もっと見る

参考文献 (23)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ