Application of the Upstream Region of a<i>Bacillus</i>Endoglucanase Gene to High-level Expression of Foreign Genes in<i>Bacillus subtilis</i>

• Sumitomo Nobuyuki

• Ozaki Katsuya

• Hitomi Jun

• Kawaminami Shunro

• Kobayashi Tohru

• Kawai Shuji

• Ito Susumu

書誌事項

タイトル別名

• Application of the Upstream Region of a Bacillus Endoglucanase Gene to High-level Expression of Foreign Genes in Bacillus subtilis.

抄録

A 0.4-kb ScaI-HpaI fragment, 199bp upstream of the structural gene for alkaline endoglucanase, from the alkalophilic Bacillus sp. KSM-64, was found to be essential for the extracellular production of the enzyme by recombinant Bacillus subtilis cells. We constructed a new vector, pHSP64 (5.5 kb), using pHY300PLK and part of the 5' region of the endoglucanase that contained a possible promoter region. Using recombinant B. subtilis cells that carried this vector, very high production of two endoglucanases and of chloramphenicol acetyltransferase was done.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 59 (11), 2172-2175, 1995

  公益社団法人 日本農芸化学会

キーワード

• Organic Chemistry
• Molecular Biology
• Applied Microbiology and Biotechnology
• General Medicine
• Biochemistry
• Analytical Chemistry
• Biotechnology

詳細情報

• CRID: 1390282681452755328
• NII論文ID: 110002677862
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.59.2172
• COI: 1:CAS:528:DyaK2MXpslyhtrc%3D
• ISSN: 13476947
• ISSN: 09168451
• PubMed: 8541661
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.59.2172
• 本文言語コード: en
• データソース種別:
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• 抄録ライセンスフラグ: 使用不可