Purification and Characterization of<i>β</i>-<scp>D</scp>-Glucosidase (<i>β</i>-<scp>D</scp>-Fucosidase) from<i>Bifidobacterium breve</i>clb Acclimated to Cellobiose

• NUNOURA Naoki

• OHDAN Kohji

• YANO Toshihiro

• YAMAMOTO Kenji

• KUMAGAI Hidehiko

書誌事項

タイトル別名

• Purification and Characterization of .BETA.-D-Glucosidase (.BETA.-D-Fucosidase) from Bifidobacterium breve clb Acclimated to Cellobiose.
• Purification and characterization of β-d-glucosidase (β-d-fucosidase) from Bifidobacterium breve clb acclimated to cellobiose
• Purification and characterization of β-D-glucosidase (β-D-fucosidase) from Bifidobacterium breve clb acclimated to cellobiose.

抄録

The β-D-glucosidase (EC. 3.2.1.21) activity of Bifidobacterium breve 203 was increased by acclimation with cellobiose, and the enzyme was purified to homogeneity from cell-free extracts of an acclimatized strain of B. breve clb, by ammonium sulfate fractionation and column chromatographies of anion-exchange, gel filtration, Gigapaite, and hydrophobic interaction. This enzyme had not only β-D-glucosidase activity but also β-D-fucosidase activity, which is specific to Bifidobacteria in intestinal flora. The molecular weight of the purified enzyme was estimated to be 47, 000-48, 000 and the enzyme was assumed to be a monomeric protein. The optimum pH and temperature of the enzyme were around 5.5 and 45°C, respectively. The enzyme was stable up to 40°C and between pH 5 and 8. The isoelectric point of the enzyme was 4.3 and the K_m values for p-nitrophenyl-β-D-glucoside and p-nitrophenyl-β-D-fucoside were 1.3 mM and 0.7 mM, respectively. This enzyme had also transferase activity for the β-D-fucosyl group but not for the β-D-glucosyl group. The N-terminal amino acid sequence of this enzyme was similar to those of β-D-glucosidase from other bacteria, actinomycetes, and plants.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 60 (2), 188-193, 1996

  公益社団法人 日本農芸化学会

キーワード

• Bifidobacterium breve
• β-D-glucosidase
• β-D-fucosidase
• acclimation
• transglycosylation

詳細情報

• CRID: 1390001206474206080
• NII論文ID: 110002677968
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.60.188
• COI: 1:CAS:528:DyaK28XhtlKntLg%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• PubMed: 9063964
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60.188
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可